Virus:Article Title: Decoding transcriptional identity in developing human sensory neurons and organoid modeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-NTRK1 R and D Systems Cat#AF1056; RRID: AB_2283049 mouse monoclonal anti-RUNX1 Santa Cruz Biotechnology Cat#sc-365644; RRID: AB_10843207 rabbit monoclonal anti-NTRK3 Cell Signaling Technology Cat#3376S:RRID: AB_2155283 mouse monoclonal anti-RUNX3 Abcam Cat#ab135248; RRID: AB_2848183 Goat polyclonal anti-NTRK2 R and D Systems Cat#AF1494; RRID: AB_2155264 mouse monoclonal anti-APOE PTM Biolabs Cat# PTM-5915; RRID: AB_3665132 goat polyclonal anti-ISL1 R and D Systems Cat#AF1837; RRID: AB_2126324 rabbit polyclonal anti-ISL1 Abcam Cat#ab20670; RRID: AB_881306 rabbit monoclonal anti-MBP Abcam Cat#ab218011; RRID: AB_2895537 mouse monoclonal anti-MEIS2 Santa Cruz Biotechnology Cat#sc-81986; RRID: AB_2143037 rabbit polyclonal anti-PRPH Millipore Cat#AB1530; RRID: AB_90725 goat polyclonal anti-RET R and D Systems Cat#AF1485; RRID: AB_354820 mouse polyclonal anti-DCC R and D Systems Cat#AF844; RRID: AB_2089765 rabbit polyclonal anti-P2RY12 Sigma-Aldrich Cat#HPA014518; RRID: AB_2669027 rabbit polyclonal anti-SKOR2 Novus Cat#NBP2-14565; RRID: AB_2632379 rabbit monoclonal anti-SOX10 PTM Biolabs Cat#PTM-5363; RRID: AB_3665131 mouse monoclonal anti-Ki67 BD Biosciences Cat#550609; RRID: AB_393778 rabbit monoclonal anti-FOXO1 Cell Signaling Technology Cat#2880S; RRID: AB_2106495 rabbit monoclonal anti-SCN10A gift of Xu Zhang Lab Yang et al.73 rabbit monoclonal anti-SCN11A gift of Xu Zhang Lab Yang et al.73 Bacterial and virus strains pSLenti-U6-shRNA-CMV-EGFPF2A-Puro-WPRE This paper N/A pAAV-CAG-SomaGCaMP6s This paper N/A Biological samples human fetal DRG Beijing Anzhen Hospital N/A, materials available on request from lead contact Chemicals, peptides, and recombinant proteins 4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) Roche Cat#10236276001 Triton X-100 Sigma-Aldrich Cat#T9284 Y27632 Tocris Bioscience Cat#1254 SB431542 Tocris Bioscience Cat#1614 CHIR-99021 AbMole Cat#M1692 FGF2 PeproTech Cat#100-18B recombinant human Brain Derived Neurotrophic Factor PeproTech Cat#450-02 Glial-Derived Neurotrophic Factor PeproTech Cat#450-10 b-Nerve Growth factor Peprotech Cat#450-01 Neurotrophin-3 Peprotech Cat#450-03 Retinoic Acid Merck Cat#R2625 MEISi-2 Dihydrochloride Selleck Cat#S6062 Geltrex Gibco Cat#A1413302 Essential 8 Medium Gibco Cat#A1517001 (Continued on next page) Cell 187, 1–20.e1–e10, December 26, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Accutase Gibco Cat#A11105-01 DMEM/F12 Gibco Cat#11330-032 KSR Gibco Cat#A31815-02 Glutamax Gibco Cat#35050-061 NEAA Gibco Cat#11140-050 beta-mercaptoethanol Gibco Cat#21985-023 Pen Strep Gibco Cat#15140-122 Neurobasal medium Gibco Cat#12348-017 B-27 supplement, minus vitamin A Gibco Cat#12587010 N-2 supplement Gibco Cat#17502-048 Cal-520, AM Santa Cruz Biotechnology Cat#sc-477280 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 10x Genomics Cat#PN-1000075 Chromium Single Cell B Chip Kit 10x Genomics Cat#PN-1000074 Chromium i7 Multiplex Kit 10x Genomics Cat#PN-120262 HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat#R333 Quantitative real-time PCR was performed using ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) Vazyme Cat# Q441-02 Deposited data Human embryonic DRG single neclei RNA-seq data and hDRGO single neclei RNA-seq data This paper GEO: GSE245310; GSA: HRA005612 Original code used for data analysis This paper https://zenodo.org/doi/10.5281/ zenodo.13826706 Human adult DRG single neclei RNA-seq data Jung et al.50 GEO: GSE201654 Mouse DRG single cell RNA-seq data Sharma et al.16 GEO: GSE139088 Experimental models: Cell lines
Recombinant:Article Title: Decoding transcriptional identity in developing human sensory neurons and organoid modeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-NTRK1 R and D Systems Cat#AF1056; RRID: AB_2283049 mouse monoclonal anti-RUNX1 Santa Cruz Biotechnology Cat#sc-365644; RRID: AB_10843207 rabbit monoclonal anti-NTRK3 Cell Signaling Technology Cat#3376S:RRID: AB_2155283 mouse monoclonal anti-RUNX3 Abcam Cat#ab135248; RRID: AB_2848183 Goat polyclonal anti-NTRK2 R and D Systems Cat#AF1494; RRID: AB_2155264 mouse monoclonal anti-APOE PTM Biolabs Cat# PTM-5915; RRID: AB_3665132 goat polyclonal anti-ISL1 R and D Systems Cat#AF1837; RRID: AB_2126324 rabbit polyclonal anti-ISL1 Abcam Cat#ab20670; RRID: AB_881306 rabbit monoclonal anti-MBP Abcam Cat#ab218011; RRID: AB_2895537 mouse monoclonal anti-MEIS2 Santa Cruz Biotechnology Cat#sc-81986; RRID: AB_2143037 rabbit polyclonal anti-PRPH Millipore Cat#AB1530; RRID: AB_90725 goat polyclonal anti-RET R and D Systems Cat#AF1485; RRID: AB_354820 mouse polyclonal anti-DCC R and D Systems Cat#AF844; RRID: AB_2089765 rabbit polyclonal anti-P2RY12 Sigma-Aldrich Cat#HPA014518; RRID: AB_2669027 rabbit polyclonal anti-SKOR2 Novus Cat#NBP2-14565; RRID: AB_2632379 rabbit monoclonal anti-SOX10 PTM Biolabs Cat#PTM-5363; RRID: AB_3665131 mouse monoclonal anti-Ki67 BD Biosciences Cat#550609; RRID: AB_393778 rabbit monoclonal anti-FOXO1 Cell Signaling Technology Cat#2880S; RRID: AB_2106495 rabbit monoclonal anti-SCN10A gift of Xu Zhang Lab Yang et al.73 rabbit monoclonal anti-SCN11A gift of Xu Zhang Lab Yang et al.73 Bacterial and virus strains pSLenti-U6-shRNA-CMV-EGFPF2A-Puro-WPRE This paper N/A pAAV-CAG-SomaGCaMP6s This paper N/A Biological samples human fetal DRG Beijing Anzhen Hospital N/A, materials available on request from lead contact Chemicals, peptides, and recombinant proteins 4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) Roche Cat#10236276001 Triton X-100 Sigma-Aldrich Cat#T9284 Y27632 Tocris Bioscience Cat#1254 SB431542 Tocris Bioscience Cat#1614 CHIR-99021 AbMole Cat#M1692 FGF2 PeproTech Cat#100-18B recombinant human Brain Derived Neurotrophic Factor PeproTech Cat#450-02 Glial-Derived Neurotrophic Factor PeproTech Cat#450-10 b-Nerve Growth factor Peprotech Cat#450-01 Neurotrophin-3 Peprotech Cat#450-03 Retinoic Acid Merck Cat#R2625 MEISi-2 Dihydrochloride Selleck Cat#S6062 Geltrex Gibco Cat#A1413302 Essential 8 Medium Gibco Cat#A1517001 (Continued on next page) Cell 187, 1–20.e1–e10, December 26, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Accutase Gibco Cat#A11105-01 DMEM/F12 Gibco Cat#11330-032 KSR Gibco Cat#A31815-02 Glutamax Gibco Cat#35050-061 NEAA Gibco Cat#11140-050 beta-mercaptoethanol Gibco Cat#21985-023 Pen Strep Gibco Cat#15140-122 Neurobasal medium Gibco Cat#12348-017 B-27 supplement, minus vitamin A Gibco Cat#12587010 N-2 supplement Gibco Cat#17502-048 Cal-520, AM Santa Cruz Biotechnology Cat#sc-477280 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 10x Genomics Cat#PN-1000075 Chromium Single Cell B Chip Kit 10x Genomics Cat#PN-1000074 Chromium i7 Multiplex Kit 10x Genomics Cat#PN-120262 HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat#R333 Quantitative real-time PCR was performed using ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) Vazyme Cat# Q441-02 Deposited data Human embryonic DRG single neclei RNA-seq data and hDRGO single neclei RNA-seq data This paper GEO: GSE245310; GSA: HRA005612 Original code used for data analysis This paper https://zenodo.org/doi/10.5281/ zenodo.13826706 Human adult DRG single neclei RNA-seq data Jung et al.50 GEO: GSE201654 Mouse DRG single cell RNA-seq data Sharma et al.16 GEO: GSE139088 Experimental models: Cell lines
Derivative Assay:Article Title: Decoding transcriptional identity in developing human sensory neurons and organoid modeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-NTRK1 R and D Systems Cat#AF1056; RRID: AB_2283049 mouse monoclonal anti-RUNX1 Santa Cruz Biotechnology Cat#sc-365644; RRID: AB_10843207 rabbit monoclonal anti-NTRK3 Cell Signaling Technology Cat#3376S:RRID: AB_2155283 mouse monoclonal anti-RUNX3 Abcam Cat#ab135248; RRID: AB_2848183 Goat polyclonal anti-NTRK2 R and D Systems Cat#AF1494; RRID: AB_2155264 mouse monoclonal anti-APOE PTM Biolabs Cat# PTM-5915; RRID: AB_3665132 goat polyclonal anti-ISL1 R and D Systems Cat#AF1837; RRID: AB_2126324 rabbit polyclonal anti-ISL1 Abcam Cat#ab20670; RRID: AB_881306 rabbit monoclonal anti-MBP Abcam Cat#ab218011; RRID: AB_2895537 mouse monoclonal anti-MEIS2 Santa Cruz Biotechnology Cat#sc-81986; RRID: AB_2143037 rabbit polyclonal anti-PRPH Millipore Cat#AB1530; RRID: AB_90725 goat polyclonal anti-RET R and D Systems Cat#AF1485; RRID: AB_354820 mouse polyclonal anti-DCC R and D Systems Cat#AF844; RRID: AB_2089765 rabbit polyclonal anti-P2RY12 Sigma-Aldrich Cat#HPA014518; RRID: AB_2669027 rabbit polyclonal anti-SKOR2 Novus Cat#NBP2-14565; RRID: AB_2632379 rabbit monoclonal anti-SOX10 PTM Biolabs Cat#PTM-5363; RRID: AB_3665131 mouse monoclonal anti-Ki67 BD Biosciences Cat#550609; RRID: AB_393778 rabbit monoclonal anti-FOXO1 Cell Signaling Technology Cat#2880S; RRID: AB_2106495 rabbit monoclonal anti-SCN10A gift of Xu Zhang Lab Yang et al.73 rabbit monoclonal anti-SCN11A gift of Xu Zhang Lab Yang et al.73 Bacterial and virus strains pSLenti-U6-shRNA-CMV-EGFPF2A-Puro-WPRE This paper N/A pAAV-CAG-SomaGCaMP6s This paper N/A Biological samples human fetal DRG Beijing Anzhen Hospital N/A, materials available on request from lead contact Chemicals, peptides, and recombinant proteins 4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) Roche Cat#10236276001 Triton X-100 Sigma-Aldrich Cat#T9284 Y27632 Tocris Bioscience Cat#1254 SB431542 Tocris Bioscience Cat#1614 CHIR-99021 AbMole Cat#M1692 FGF2 PeproTech Cat#100-18B recombinant human Brain Derived Neurotrophic Factor PeproTech Cat#450-02 Glial-Derived Neurotrophic Factor PeproTech Cat#450-10 b-Nerve Growth factor Peprotech Cat#450-01 Neurotrophin-3 Peprotech Cat#450-03 Retinoic Acid Merck Cat#R2625 MEISi-2 Dihydrochloride Selleck Cat#S6062 Geltrex Gibco Cat#A1413302 Essential 8 Medium Gibco Cat#A1517001 (Continued on next page) Cell 187, 1–20.e1–e10, December 26, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Accutase Gibco Cat#A11105-01 DMEM/F12 Gibco Cat#11330-032 KSR Gibco Cat#A31815-02 Glutamax Gibco Cat#35050-061 NEAA Gibco Cat#11140-050 beta-mercaptoethanol Gibco Cat#21985-023 Pen Strep Gibco Cat#15140-122 Neurobasal medium Gibco Cat#12348-017 B-27 supplement, minus vitamin A Gibco Cat#12587010 N-2 supplement Gibco Cat#17502-048 Cal-520, AM Santa Cruz Biotechnology Cat#sc-477280 Critical commercial assays Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 10x Genomics Cat#PN-1000075 Chromium Single Cell B Chip Kit 10x Genomics Cat#PN-1000074 Chromium i7 Multiplex Kit 10x Genomics Cat#PN-120262 HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat#R333 Quantitative real-time PCR was performed using ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) Vazyme Cat# Q441-02 Deposited data Human embryonic DRG single neclei RNA-seq data and hDRGO single neclei RNA-seq data This paper GEO: GSE245310; GSA: HRA005612 Original code used for data analysis This paper https://zenodo.org/doi/10.5281/ zenodo.13826706 Human adult DRG single neclei RNA-seq data Jung et al.50 GEO: GSE201654 Mouse DRG single cell RNA-seq data Sharma et al.16 GEO: GSE139088 Experimental models: Cell lines
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